Plasmid Preparation:Article Title: Efficient translation of mRNAs in influenza A virus-infected cells is independent of the viral 5' untranslated region.
Article Snippet: To con- truct the pNP-Luc-NP plasmid, the pSP64Poly(A) plasid (Promega), which was modified to contain an Xmn1 estriction site in place of an EcoRI site, was digested ith HindII and SacI, and the following three DNA seuences were then ligated into this linearized plasmid: 1) the 59-UTR of influenza virus NP mRNA containing indII and NcoI ends, obtained by annealing two oligoucleotides (48 and 51 nucleotides in length) of the ppropriate sequence; (2) the firefly luciferase ORF con- aining NcoI and NotI ends; and (3) the 39-UTR of influenza virus NP mRNA containing NotI and SacI ends, btained by annealing two oligonucleotides of the apropriate sequence.
Modification:Article Title: Efficient translation of mRNAs in influenza A virus-infected cells is independent of the viral 5' untranslated region.
Article Snippet: To con- truct the pNP-Luc-NP plasmid, the pSP64Poly(A) plasid (Promega), which was modified to contain an Xmn1 estriction site in place of an EcoRI site, was digested ith HindII and SacI, and the following three DNA seuences were then ligated into this linearized plasmid: 1) the 59-UTR of influenza virus NP mRNA containing indII and NcoI ends, obtained by annealing two oligoucleotides (48 and 51 nucleotides in length) of the ppropriate sequence; (2) the firefly luciferase ORF con- aining NcoI and NotI ends; and (3) the 39-UTR of influenza virus NP mRNA containing NotI and SacI ends, btained by annealing two oligonucleotides of the apropriate sequence.
Virus:Article Title: Efficient translation of mRNAs in influenza A virus-infected cells is independent of the viral 5' untranslated region.
Article Snippet: To con- truct the pNP-Luc-NP plasmid, the pSP64Poly(A) plasid (Promega), which was modified to contain an Xmn1 estriction site in place of an EcoRI site, was digested ith HindII and SacI, and the following three DNA seuences were then ligated into this linearized plasmid: 1) the 59-UTR of influenza virus NP mRNA containing indII and NcoI ends, obtained by annealing two oligoucleotides (48 and 51 nucleotides in length) of the ppropriate sequence; (2) the firefly luciferase ORF con- aining NcoI and NotI ends; and (3) the 39-UTR of influenza virus NP mRNA containing NotI and SacI ends, btained by annealing two oligonucleotides of the apropriate sequence.
Sequencing:Article Title: Efficient translation of mRNAs in influenza A virus-infected cells is independent of the viral 5' untranslated region.
Article Snippet: To con- truct the pNP-Luc-NP plasmid, the pSP64Poly(A) plasid (Promega), which was modified to contain an Xmn1 estriction site in place of an EcoRI site, was digested ith HindII and SacI, and the following three DNA seuences were then ligated into this linearized plasmid: 1) the 59-UTR of influenza virus NP mRNA containing indII and NcoI ends, obtained by annealing two oligoucleotides (48 and 51 nucleotides in length) of the ppropriate sequence; (2) the firefly luciferase ORF con- aining NcoI and NotI ends; and (3) the 39-UTR of influenza virus NP mRNA containing NotI and SacI ends, btained by annealing two oligonucleotides of the apropriate sequence.
Luciferase:Article Title: Efficient translation of mRNAs in influenza A virus-infected cells is independent of the viral 5' untranslated region.
Article Snippet: To con- truct the pNP-Luc-NP plasmid, the pSP64Poly(A) plasid (Promega), which was modified to contain an Xmn1 estriction site in place of an EcoRI site, was digested ith HindII and SacI, and the following three DNA seuences were then ligated into this linearized plasmid: 1) the 59-UTR of influenza virus NP mRNA containing indII and NcoI ends, obtained by annealing two oligoucleotides (48 and 51 nucleotides in length) of the ppropriate sequence; (2) the firefly luciferase ORF con- aining NcoI and NotI ends; and (3) the 39-UTR of influenza virus NP mRNA containing NotI and SacI ends, btained by annealing two oligonucleotides of the apropriate sequence.
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